Screw-shaped Contaminant of Distilled Water.

FIG. 9. Direct contact photography of Armillaria mellea hyphae by its own light. Kodak Microfile, 3-hr exposure. Negative photomicrographed; figutre constitutes a negative print. Arrows indicate clamp connections. X 1,200. FIG. 10. Direct contact photography of Armillaria mellea by its own light. Polaroid Type 55 P/N filn, 30-min exposure. Negative photomicrographed on Microfile film, figuire constitutes a negative print. X 1,000. for successful exposures with Microfile or Adox KB21. Thereafter, either the agar dried up or the emulsion peeled off the film backing. With the Polaroid film, where unfortunately the grain is less even, a 1-hr exposure was sufficient. After the film was processed, the exposed areas were examined microscopically. The growth pattern and mycelial distribution of the edge of a growing culture where the hyphae are distinct, separate, and in one layer were clearly duplicated in the emulsion grain. Figures 9, 10, and 11 show the amount of detail observable. The negatives were then photomicrographed with Microfile film and the pictures enlarged when printed. In Fig. 9 the arrows indicate clamp connections, and in Fig. 11 the branching pattern is readily evident. Finer structural details cannot be made out, and it remains to find an emulsion of equivalent speed, but with considerably smaller even grains, before the area of light emission within the cell can be determined. For the present, it appears that light emission occurs throughout the cell.